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  4. Mouse Interferon α1,IFN-α1 ELISA Kit

Mouse Interferon α1,IFN-α1 ELISA Kit

  • 中文名稱：
    小鼠α1干擾素(IFN-α1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08638m-IS
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    1.25pg/mL-80pg/mL
  • 靈敏度：
    0.625pg/mL
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 促銷(xiāo)：
  • 其他：

產(chǎn)品詳情

  • 別名：
    Ifna1 ELISA Kit; Interferon alpha-1 ELISA Kit; IFN-alpha-1 ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 反應(yīng)時(shí)間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-α1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    91

    Range %

    84-97

    1:2

    Average %

    102

    Range %

    98-107

    1:4

    Average %

    97

    Range %

    92-103

    1:8

    Average %

    93

    Range %

    89-98

  • 回收率：

    The recovery of mouse IFN-α1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    98

    91-104

    EDTA plasma (n=4)

    91

    83-99

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    80

    2.628

    2.634

    2.631

    2.504

    40

    1.672

    1.667

    1.670

    1.543

    20

    1.015

    1.028

    1.022

    0.895

    10

    0.616

    0.629

    0.622

    0.496

    5

    0.360

    0.371

    0.365

    0.239

    2.5

    0.290

    0.283

    0.286

    0.160

    1.25

    0.191

    0.187

    0.189

    0.062

    0

    0.129

    0.125

    0.127

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
  • 基因功能參考文獻(xiàn)：
    1. These findings also identify STAT3 as a therapeutic target against viral infection and highlight it as an essential pathway component for endogenous and therapeutic IFN-alpha responsiveness. PMID: 27988795
    2. the close association between the increased proportion of CD180-negative B cells and the activation of IFN-alpha signaling in Systemic lupus erythematosus, is reported. PMID: 26277892
    3. IL-12 and IFN-alpha differentially program CD8 T cells to re-express distinct levels of PD-1 upon re-encountering Ag, resulting in IL-12-stimulated cells being less susceptible to exhaustion in the face of sustained tumor Ag. PMID: 23804712
    4. The expansion of CD8 T cells depends on type I IFN, type I IFN and IL-12 or is largely independent of the two cytokines. PMID: 22815848
    5. A spontaneous genomic duplication and frameshift mutation in the guanine exchange factor dedicator of cytokinesis 2 (Dock2) that has arisen in at least a subset of circulating Irf5(-/-) mice and inadvertently been bred to homozygosity. PMID: 22431588
    6. Knockdown of endogenous guanylate binding protein (GBP)4 increases IRF7-mediated IFN-alpha production, whereas overexpression of GBP4 has the opposite effect. PMID: 22095711
    7. protects against antigen-induced arthritis PMID: 21469099
    8. Data show that similar T cell expansion and serum IgG responses were observed in adenovirus (Adv)-IFN-treated WT and BAFF-deficient mice despite their disparate pathological and clinical responses. PMID: 21383240
    9. Studies in both mice and humans have demonstrated a role for IFN-alpha/beta in directly influencing the fate of both CD4(+) and CD8(+) T cells during the initial phases of antigen recognition. PMID: 21320124
    10. IFN-alpha enhances both the induction and maintenance of programmed cell death (PD)-1 expression on T cell receptor-engaged primary mouse T cells through association of IFN-responsive factor 9 (IRF9) with the IFN stimulation response element. PMID: 21263073
    11. This article reports the production of interferon alpha/beta (IFN-alpha/beta) by SJL/J mouse brain astrocyte cultures infected with Theiler's murine encephalomyelitis virus (TMEV). PMID: 20038206
    12. TLR ligands facilitate antibody response by inducing type I interferon (IFN), which in turn elicits rapid and significant amounts of antigen-specific IgG2c. PMID: 20566717
    13. These results demonstrate that mouse hepatitis virus is recognized by both RIG-I and MDA5 and induces IFN-alpha/beta through the activation of the IRF-3 signaling pathway. PMID: 20427526
    14. Sufficient IFN-alpha production by plasmacytoid dentritic cells is an important determinant of vaccine efficacy. PMID: 20065291
    15. B cell-mediated type I IFN induction depended on the transcription factor IRF3. PMID: 17925397
    16. The involvement of IRF1 and IRF2 in TLR2-mediated responses, was studied. PMID: 18652890
    17. Report that tumor-targeted interferon-alpha1 delivery by Tie2-expressing monocytes inhibits tumor growth and metastasis. PMID: 18835032
    18. It is likely that IFN-alpha plays a complex role in the etiology of type 1 diabetes. PMID: 19120292
    19. Collectively, these findings demonstrate that constitutive and LPS-induced type I IFN play significant roles in regulating the differences in phenotype and function between BMM and GM-BMM. PMID: 19406830
    20. Recombinant IFN-alpha treatment promotes monocyte chemoattractant protein (MCP)-1, MCP-5, and, to a lesser extent, MCP-3 production in the bone marrow during murocytomegalovirus infection. PMID: 19620305

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Alpha/beta interferon family
  • 數(shù)據(jù)庫(kù)鏈接：


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