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  4. Mouse Immunoglobulin E,IgE ELISA Kit

Mouse Immunoglobulin E,IgE ELISA Kit

  • 中文名稱：
    小鼠免疫球蛋白E(IgE)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07983m-IS
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    15.6pg/mL-1000pg/mL
  • 靈敏度：
    7.8pg/mL
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 縮寫：
    IgE
  • 種屬：
    Mus musculus (Mouse)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:200

    Average %

    87

    Range %

    83-92

    1:400

    Average %

    90

    Range %

    85-96

    1:800

    Average %

    96

    Range %

    89-103

    1:1600

    Average %

    93

    Range %

    86-100

  • 回收率：

    The recovery of mouse IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    91

    83-98

    EDTA plasma (n=4)

    97

    90-105

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    1000

    2.796

    2.634

    2.715

    2.603

    500

    2.287

    2.167

    2.227

    2.116

    250

    1.521

    1.608

    1.565

    1.453

    125

    0.863

    0.899

    0.881

    0.769

    62.5

    0.634

    0.621

    0.628

    0.516

    31.2

    0.416

    0.403

    0.409

    0.298

    15.6

    0.280

    0.284

    0.282

    0.170

    0

    0.108

    0.115

    0.112

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days.

產(chǎn)品評價



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