Mouse High density lipoprotein,HDL ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠高密度脂蛋白(HDL)酶聯(lián)免疫試劑盒（CSB-E12840m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的HDL含量。HDL即高密度脂蛋白，它具有抗動(dòng)脈粥樣硬化作用，在膽固醇逆向轉(zhuǎn)運(yùn)等過(guò)程中發(fā)揮關(guān)鍵作用。研究聚焦其提升機(jī)制，比如激活相關(guān)信號(hào)通路、調(diào)節(jié)關(guān)鍵酶活性，以提高HDL水平和功能，進(jìn)而防治心血管疾病。試劑盒檢測(cè)范圍為31.25 ng/mL-2000 ng/mL，適用于科研領(lǐng)域中對(duì)小鼠模型脂代謝機(jī)制、心血管疾病病理研究或藥物干預(yù)效果的評(píng)價(jià)；為探索HDL在生理及疾病狀態(tài)下的功能提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
縮寫：

HDL
種屬：

Mus musculus (Mouse)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse HDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	88
	Range %	84-92
1:2000	Average %	94
	Range %	90-98
1:4000	Average %	99
	Range %	95-104
1:8000	Average %	102
	Range %	96-108

回收率：

The recovery of mouse HDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	100	95-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.