Human β-hexosaminidase A,β-Hex A ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人β氨基己糖苷酶A(β-Hex A)酶聯(lián)免疫試劑盒（CSB-E09462h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的βHex A含量。βHex A是一種重要靶點(diǎn)。它在某些疾病發(fā)病機(jī)制中起關(guān)鍵作用，如泰-薩克斯病等。研究機(jī)制方面，主要聚焦于其在細(xì)胞內(nèi)代謝產(chǎn)物的清除過(guò)程，通過(guò)解析其結(jié)構(gòu)與功能，試圖尋找調(diào)節(jié)它的方法，以研發(fā)治療相關(guān)疾病的藥物。試劑盒檢測(cè)范圍為1.56 mIU/mL-100 mIU/mL，該產(chǎn)品適用于科研領(lǐng)域中對(duì)β - Hex A表達(dá)水平的定量分析，可用于細(xì)胞模型、動(dòng)物實(shí)驗(yàn)或人體生物樣本中酶活性變化的研究，為探索溶酶體貯積癥發(fā)病機(jī)制、代謝通路調(diào)控或潛在治療策略的體外評(píng)估提供高效工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

beta-hexosaminidase A, beta-Hex A
縮寫(xiě)：

βHex A
種屬：

Homo sapiens (Human)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Neuroscience
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human β-Hex A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
Range %	85-94
1:2	Average %	93
Range %	88-94
1:4	Average %	105
Range %	100-112
1:8	Average %	86
Range %	80-92

回收率：

The recovery of human β-Hex A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-102
EDTA plasma (n=4)	92	88-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.