Human β-Thromboglobulin,β-TG ELISA Kit

產(chǎn)品詳情

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文獻(xiàn)引用 (2) 說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人β血小板球蛋白/β血栓環(huán)蛋白(β-TG)酶聯(lián)免疫試劑盒（CSB-E07886h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的β-TG含量。β-TG即β-血小板球蛋白，是血小板α顆粒特有的蛋白質(zhì)。其釋放水平可反映血小板的活化程度。研究中常用它作為體內(nèi)血小板激活的指標(biāo)，探究其在血栓形成、炎癥反應(yīng)等病理過(guò)程中的作用及機(jī)制，以尋找相關(guān)疾病診斷和治療的新靶點(diǎn)。試劑盒檢測(cè)范圍為0.78 ng/mL-50 ng/mL，本試劑盒適用于基礎(chǔ)科研中對(duì)血小板功能評(píng)估、血栓性疾病機(jī)制研究、藥物干預(yù)效果分析等場(chǎng)景，例如通過(guò)檢測(cè)患者血漿中β-TG濃度變化探索抗血小板藥物的作用機(jī)理，或利用組織勻漿分析β-TG在局部病理微環(huán)境中的表達(dá)特征本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
別名：

beta-Thromboglobulin, beta-TG
縮寫：

β-TG
種屬：

Homo sapiens (Human)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human β-TG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-97
1:2	Average %	90
	Range %	85-95
1:4	Average %	103
	Range %	97-109
1:8	Average %	84
	Range %	80-89

回收率：

The recovery of human β-TG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-101
EDTA plasma (n=4)	95	90-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.