Human type Ⅱ collagen helical peptide,HELIX-Ⅱ ELISA Kit
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中文名稱:人Ⅱ型膠原螺旋肽(HELIX-Ⅱ)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E09708h
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樣本類型:serum, plasma, urine
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檢測(cè)范圍:1.25 ng/mL-80 ng/mL
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靈敏度:0.312 ng/mL
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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促銷:
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人Ⅱ型膠原螺旋肽(HELIX-Ⅱ)酶聯(lián)免疫試劑盒(CSB-E09708h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、尿液樣本中的HELIXⅡ含量。試劑盒檢測(cè)范圍為1.25 ng/mL-80 ng/mL,Ⅱ型膠原是關(guān)節(jié)軟骨的主要結(jié)構(gòu)蛋白,其螺旋結(jié)構(gòu)域(HELIX - Ⅱ)的降解產(chǎn)物可作為軟骨代謝異常的分子標(biāo)志物,在骨關(guān)節(jié)疾病機(jī)制研究、軟骨損傷動(dòng)態(tài)監(jiān)測(cè)及藥物干預(yù)效果評(píng)價(jià)等領(lǐng)域具有重要科研價(jià)值。研究人員可通過(guò)該產(chǎn)品探究骨關(guān)節(jié)炎、類風(fēng)濕性關(guān)節(jié)炎等疾病進(jìn)程中軟骨基質(zhì)降解的動(dòng)態(tài)變化,或用于篩選調(diào)節(jié)軟骨代謝的潛在化合物,為相關(guān)領(lǐng)域的分子機(jī)制研究和治療策略開(kāi)發(fā)提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
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別名:type 2 collagen helical peptide,HELIX-2,_x000D_ type II collagen helical peptide,HELIX-II
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縮寫:HELIXⅡ
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種屬:Homo sapiens (Human)
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反應(yīng)時(shí)間:1-5h
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上樣量:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Others
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human HELIX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 90-99 1:2 Average % 89 Range % 83-94 1:4 Average % 99 Range % 94-103 1:8 Average % 104 Range % 100-112 -
回收率:
The recovery of human HELIX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 84 80-88 EDTA plasma (n=4) 91 86-97 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected 80 2.178 2.114 2.146 2.043 40 1.674 1.581 1.628 1.525 20 1.116 1.061 1.089 0.986 10 0.626 0.603 0.615 0.512 5 0.423 0.407 0.415 0.312 2.5 0.252 0.239 0.246 0.143 1.25 0.193 0.184 0.189 0.086 0 0.104 0.101 0.103 -
數(shù)據(jù)處理:
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貨期:3-5 working days
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