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Human platelet-derived growth factor CC (PDGF-CC) ELISA Kit

  • 中文名稱:
    人血小板衍生生長因子CC(PDGF-CC)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E13241h
  • 樣本類型:
    serum, cell culture supernates, urine, tissue homogenates
  • 檢測范圍:
    0.156 ng/mL-10 ng/mL
  • 靈敏度:
    0.039 ng/mL
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人血小板衍生生長因子CC(PDGF-CC)酶聯(lián)免疫試劑盒(CSB-E13241h)為雙抗夾心法ELISA試劑盒,定量檢測血清、組織培養(yǎng)上清液、尿液、組織勻漿樣本中的PDGF-CC含量。PDGF-CC 即血小板衍生生長因子 CC,在細(xì)胞生長、增殖等生理過程中發(fā)揮作用。其異常表達(dá)與腫瘤、纖維化等疾病的發(fā)生發(fā)展相關(guān)。研究機(jī)制主要聚焦于其信號通路,阻斷該信號有望為相關(guān)疾病治療提供方向,目前是研究熱門靶點(diǎn)。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL,科研人員可通過該產(chǎn)品開展PDGF-CC在疾病模型中的表達(dá)水平監(jiān)測、藥物干預(yù)效果評估或信號通路機(jī)制研究,為心血管生物學(xué)、腫瘤微環(huán)境及再生醫(yī)學(xué)等領(lǐng)域的體外實(shí)驗(yàn)提供可靠數(shù)據(jù)支持。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    PDGF-CC
  • 種屬:
    Homo sapiens (Human)
  • 反應(yīng)時(shí)間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Cardiovascular
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human PDGF-CC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %92
    Range %88-96
    1:2Average %95
    Range %92-99
    1:4Average %94
    Range %89-101
    1:8Average %89
    Range %84-95
  • 回收率:
    The recovery of human PDGF-CC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9691-102
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.308 2.418 2.363 2.260
    51.887 2.032 1.960 1.857
    2.51.452 1.423 1.438 1.335
    1.250.782 0.821 0.802 0.699
    0.6250.477 0.497 0.487 0.384
    0.3120.325 0.346 0.336 0.233
    0.1560.215 0.205 0.210 0.107
    00.104 0.101 0.103  
     
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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