Human cytokeratin fragment antigen 21-1,CYFRA21-1 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人細(xì)胞角蛋白21-1片段(CYFRA21-1)酶聯(lián)免疫試劑盒（CSB-E09606h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的CYFRA21-1含量。CYFRA21-1是一種細(xì)胞角蛋白片段，廣泛分布于正常組織中。當(dāng)細(xì)胞癌變時(shí)，角蛋白被降解并釋放到血液中，CYFRA21-1水平升高。它作為肺癌，尤其是鱗狀細(xì)胞癌的標(biāo)志物，可用于診斷和預(yù)后評(píng)估。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，本產(chǎn)品適用于基礎(chǔ)醫(yī)學(xué)研究中腫瘤標(biāo)志物表達(dá)水平檢測(cè)、疾病機(jī)制探索或藥物開發(fā)過程中的生物標(biāo)志物動(dòng)態(tài)監(jiān)測(cè)，為體外實(shí)驗(yàn)提供血清/血漿樣本中CYFRA21 - 1濃度的可靠數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

CYFRA21-1
種屬：

Homo sapiens (Human)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CYFRA21-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-90
1:2	Average %	98
	Range %	91-105
1:4	Average %	99
	Range %	94-110
1:8	Average %	92
	Range %	86-99

回收率：

The recovery of human CYFRA21-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	80-98
EDTA plasma (n=4)	97	90-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.