Human alzheimer-associated neuronal thread protein (AD7C-NTP) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人阿爾茨海默病相關(guān)神經(jīng)絲蛋白(AD7C-NTP)酶聯(lián)免疫試劑盒（CSB-EQ027774HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AD7C-NTP含量。試劑盒檢測范圍為93.75 pg/mL-6000 pg/mL，靈敏度為23.43 pg/mL。適用于科研領(lǐng)域中對阿爾茨海默病發(fā)病機(jī)制的基礎(chǔ)研究、藥物開發(fā)過程中生物標(biāo)志物動態(tài)監(jiān)測，或探索腦脊液外其他體液樣本中AD7C-NTP的表達(dá)水平。支持研究者對多種生物樣本進(jìn)行批量分析，為神經(jīng)退行性疾病模型構(gòu)建、病理分子網(wǎng)絡(luò)解析等實(shí)驗(yàn)提供可靠數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

AD7C-NTP
種屬：

Homo sapiens (Human)
反應(yīng)時間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of Human AD7C-NTP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	97
	Range %	93-101
1:40	Average %	93
	Range %	86-97
1:80	Average %	93
	Range %	87-97
1:160	Average %	102
	Range %	98-106

回收率：

The recovery of Human AD7C-NTP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	87	82-93

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

最新研究進(jìn)展：

AD7C-NTP是一種神經(jīng)元特異性蛋白質(zhì)，最新研究表明，其可能與阿爾茨海默病的發(fā)生和發(fā)展有關(guān)。研究人員發(fā)現(xiàn)，AD7C-NTP的水平在阿爾茨海默病患者中顯著升高，可能是一種早期的生物標(biāo)志物。