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  4. Human Spondin-1(SPON1) ELISA kit

Human Spondin-1(SPON1) ELISA kit

  • 中文名稱：
    人脊椎蛋白-1(SPON1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL022599HU-IS
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.312ng/mL-20ng/mL
  • 靈敏度：
    0.156ng/mL
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 別名：
    SPON1 ELISA kit; KIAA0762 ELISA kit; VSGPSpondin-1 ELISA kit; F-spondin ELISA kit; Vascular smooth muscle cell growth-promoting factor ELISA kit
  • 縮寫：
    SPON1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 反應(yīng)時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human SPON1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    93

    Range %

    88-99

    1:2

    Average %

    101

    Range %

    94-108

    1:4

    Average %

    98

    Range %

    91-105

    1:8

    Average %

    90

    Range %

    85-95

  • 回收率：

    The recovery of human SPON1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    96

    89-103

    EDTA plasma (n=4)

    91

    85-97

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    20

    3.068

    2.934

    3.001

    2.840

    10

    2.078

    1.967

    2.022

    1.862

    5

    1.183

    1.128

    1.155

    0.995

    2.5

    0.666

    0.641

    0.654

    0.493

    1.25

    0.449

    0.431

    0.440

    0.280

    0.625

    0.305

    0.293

    0.299

    0.138

    0.312

    0.228

    0.217

    0.223

    0.062

    0

    0.164

    0.157

    0.161

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days.

產(chǎn)品評價

靶點詳情

  • 功能：
    Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS. Major factor for vascular smooth muscle cell.
  • 基因功能參考文獻：
    1. gene expression experiment revealed that CYP2B6, SPON1, and GSG1L can be activated concomitantly through a constitutive androstane receptor (CAR) activation pathway PMID: 27010727
    2. SPON1 promotes metastatic progression through Fak and Src dependent pathway in human osteosarcoma. PMID: 26032498
    3. These data suggest that SPON1 may be associated with the differential rate of cognitive decline in Alzheimer disease. PMID: 23535033
    4. High SPON1 expression is associated with high-grade gliomas. PMID: 24158112
    5. Genome-wide scan of healthy human connectome discovers SPON1 gene variant influencing dementia severity PMID: 23471985
    6. downregulates osteoclast recruitment to the root side of periodontal tissue via low-density lipoprotein receptor-related protein 8 and inhibits differentiation of osteoclastic precursors PMID: 21488757
    7. These findings indicate that F-spondin regulates the differentiation of human cementoblast-like cells via BMP7 expression. PMID: 22244873
    8. The unique feature of F-spondin FS domain is the presence of three disulfide bonds associated with the N- and C-termini of the domain and a highly conserved N-linked glycosylation site. PMID: 21569239
    9. expression profile of VSGP/F-spondin identifies this molecule as a potential diagnostic marker or target for developing therapeutic strategies to treat ovarian carcinoma PMID: 16103746
    10. identified F-spondin as a promoting factor for cementoblastic differentiation PMID: 16965763
    11. The crystal structure of the reelin-N domain resolved to 2.0 A reveals a variant immunoglobulin-like fold and potential heparin-binding sites. PMID: 18602404
    12. These studies identify F-spondin as a novel protein in osteoarthritis cartilage, where it may act in situ at lesional areas to activate latent TGF-beta and induce cartilage degradation. PMID: 18780763

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  • 亞細胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 組織特異性：
    Highest expression in lung, lower expression in brain, heart, kidney, liver and testis, and lowest expression in pancreas, skeletal muscle and ovary. Not expressed in spleen.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 11252

    OMIM: 604989

    KEGG: hsa:10418

    UniGene: Hs.643864



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