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Human Phospholipidase A2,PLA2 ELISA Kit

  • 中文名稱:
    人磷脂酶A2(PLA2)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E08110h
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    0.45 ng/mL-30 ng/mL
  • 靈敏度:
    0.11 ng/mL
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人磷脂酶A2(PLA2)酶聯(lián)免疫試劑盒(CSB-E08110h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的PLA2G1B含量。PLA2G1B是一種磷脂酶A2。它在脂質(zhì)代謝、炎癥反應(yīng)等過(guò)程中起關(guān)鍵作用。研究聚焦其水解磷脂產(chǎn)生脂肪酸和溶血磷脂機(jī)制,探索其在代謝性疾病、炎癥相關(guān)疾病中的作用,有望為相關(guān)疾病藥物研發(fā)提供新靶點(diǎn)與思路。試劑盒檢測(cè)范圍為0.45 ng/mL-30 ng/mL,能夠支持基礎(chǔ)醫(yī)學(xué)研究中PLA2相關(guān)信號(hào)通路機(jī)制探索,適用于心血管疾病模型、神經(jīng)退行性疾病模型或代謝綜合征等領(lǐng)域的蛋白表達(dá)水平檢測(cè),也可用于體外藥物篩選實(shí)驗(yàn)中PLA2活性調(diào)控化合物的作用效果評(píng)估,為相關(guān)生物標(biāo)志物研究及新型治療策略開(kāi)發(fā)提供實(shí)驗(yàn)工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名:
    Group IB phospholipase A2 ELISA Kit; Group IB secretory phospholipase A2 ELISA Kit; MGC119834 ELISA Kit; MGC119835 ELISA Kit; PA21B_HUMAN ELISA Kit; Phosphatidylcholine 2 acylhydrolase ELISA Kit; Phosphatidylcholine 2-acylhydrolase 1B ELISA Kit; Phospholipase A2 ELISA Kit; Phospholipase A2 group IB (pancreas) ELISA Kit; Phospholipase A2 group IB ELISA Kit; PLA 2 ELISA Kit; PLA2 ELISA Kit; PLA2A ELISA Kit; PLA2G1B ELISA Kit; PPLA 2 ELISA Kit; PPLA2 ELISA Kit; sPLA2IB ELISA Kit
  • 縮寫(xiě):
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 反應(yīng)時(shí)間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Metabolism
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human PLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:5Average %93
    Range %88-97
    1:10Average %104
    Range %100-109
    1:20Average %95
    Range %91-99
    1:40Average %85
    Range %81-92
  • 回收率:
    The recovery of human PLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9288-96
    EDTA plasma (n=4)9793-101
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    302.534 2.452 2.493 2.384
    151.652 1.576 1.614 1.505
    7.51.066 1.004 1.035 0.926
    3.750.578 0.561 0.570 0.461
    1.80.387 0.401 0.394 0.285
    0.90.238 0.245 0.242 0.133
    0.450.179 0.187 0.183 0.074
    00.105 0.112 0.109  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Secretory calcium-dependent phospholipase A2 that primarily targets dietary phospholipids in the intestinal tract. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. May play a role in the biosynthesis of N-acyl ethanolamines that regulate energy metabolism and inflammation in the intestinal tract. Hydrolyzes N-acyl phosphatidylethanolamines to N-acyl lysophosphatidylethanolamines, which are further cleaved by a lysophospholipase D to release N-acyl ethanolamines. May act in an autocrine and paracrine manner. Upon binding to the PLA2R1 receptor can regulate podocyte survival and glomerular homeostasis. Has anti-helminth activity in a process regulated by gut microbiota. Upon helminth infection of intestinal epithelia, directly affects phosphatidylethanolamine contents in the membrane of helminth larvae, likely controlling an array of phospholipid-mediated cellular processes such as membrane fusion and cell division while providing for better immune recognition, ultimately reducing larvae integrity and infectivity.
  • 基因功能參考文獻(xiàn):
    1. sPLA2-IB was correlated with the level of proteinuria in membranous nephropathy patients suggesting to be a potential biomarker for monitoring disease severity and therapeutic effects of both primary membranous nephropathy and secondary membranous nephropathy. PMID: 29649452
    2. The sPLA2 IB-PLA2R interaction stimulated podocyte apoptosis through activating ERK1/2 and cPLA2alpha and through increasing the podocyte AA content PMID: 25335547
    3. TNF-alpha-induced cPLA2 expression and PGE2 release were mediated through a Jak2/PDGFR/PI3K/Akt/p42/p44 MAPK/Elk-1 pathway in human lung epithelial cells. PMID: 24441870
    4. Exogenously added sPLA(2)-IB decreases phagocytosis of photoreceptor outer segments regardless of enzymatic activity. PMID: 22680611
    5. stimulation of three isoforms of PLA2 by thapsigargin liberates free AA that, in turn, induces capacitative calcium influx in human T-cells PMID: 12423354
    6. results indicate a selective sorting of a cell-derived cPLA2 during human cytomegalovirus maturation, which is further required for infectivity PMID: 15220446
    7. Group IB phospholipase A2 (PLA2G1B) stimulates leukotriene B4 (LTB4) production in the absence of cytochalasin B in human neutrophils. PMID: 16005851
    8. Here, we report sPLA2-IB in rat and human brain as well as in neurons in primary culture. The distribution of sPLA2-IB seems to be mainly neuronal, with the highest abundance occurring in the cerebral cortex and hippocampus. PMID: 16392040
    9. a critical regulatory role of arachidonate reacylation that limits leukotriene biosynthesis in concert with 5-lipoxygenase and cytosolic phospholipase A(2)alpha activation PMID: 16495221
    10. Results describe the structural basis for bile salt inhibition of pancreatic phospholipase A2. PMID: 17434532
    11. MMP-2/9 production is regulated by sPLA2-IB acting as a receptor ligand to activate cPLA2 PMID: 17981679
    12. pro-hG1B forms a trimer and PROP occupies the catalytic cavity and can be self-cleaved at 37 degrees C; A new membrane-bound surface and activation mechanism are proposed based on the trimeric model of pro-hG1B PMID: 19297324

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  • 亞細(xì)胞定位:
    Secreted. Note=Secreted from pancreatic acinar cells in its inactive form.
  • 蛋白家族:
    Phospholipase A2 family
  • 組織特異性:
    Selectively expressed in pancreas, lung, liver and kidney. Also detected at lower levels in ovary and testis.
  • 數(shù)據(jù)庫(kù)鏈接:

    HGNC: 9030

    OMIM: 172410

    KEGG: hsa:5319

    STRING: 9606.ENSP00000312286

    UniGene: Hs.992



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