Human Nesfatin-1 ELISA Kit

產(chǎn)品詳情

文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人神經(jīng)肽Nesfatin-1酶聯(lián)免疫試劑盒（CSB-E15050h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Nesfatin-1含量。Nesfatin-1是一個值得關(guān)注的靶點。它在體內(nèi)廣泛分布，與能量代謝、進食調(diào)節(jié)等生理過程密切相關(guān)。目前研究聚焦于其作用機制，如通過與特定受體結(jié)合，影響神經(jīng)信號傳導(dǎo)，調(diào)節(jié)代謝相關(guān)激素分泌，為肥胖、代謝疾病治療帶來新思路。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于代謝疾病模型研究、肥胖相關(guān)神經(jīng)內(nèi)分泌機制探索，或通過檢測腦組織、外周血等樣本動態(tài)觀察Nesfatin-1在應(yīng)激狀態(tài)下的表達(dá)變化，為研究能量代謝調(diào)控、神經(jīng)肽信號通路提供可靠工具，滿足基礎(chǔ)科研中對低豐度靶標(biāo)的高效檢測需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

Nesfatin-1
種屬：

Homo sapiens (Human)
反應(yīng)時間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Nesfatin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	96
	Range %	80-105
1:10	Average %	99
	Range %	91-111
1:20	Average %	104
	Range %	92-112
1:40	Average %	93
	Range %	86-100

回收率：

The recovery of human Nesfatin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	97	90-107

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.