日本高潮视频在线观看-亚洲中文字幕永不卡-精品亚洲一区二区三区-伦理片夜夜躁狠狠躁日日躁-日本最新免费不卡二区-国产精品口爆一区二区三区-av一区二区三区高清-大鸡巴疯狂抽插小穴视频-日韩中文国产在线观看免费视频

Your Good Partner in Biology Research

Human Hyaluronic acid,HA ELISA Kit

  • 中文名稱:
    人透明質(zhì)酸(HA)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E04805h
  • 樣本類型:
    serum, plasma, cerebrospinal fluid (CSF), tissue homogenates
  • 檢測范圍:
    0.156 ng/mL-10 ng/mL
  • 靈敏度:
    0.039 ng/mL
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
        人透明質(zhì)酸 (Hyaluronic Acid, HA) 酶聯(lián)免疫試劑盒 (貨號: CSB-E04805h)采用雙抗體夾心法 ELISA 技術(shù),專為定量檢測人血清、血漿、腦脊液(CSF)及組織勻漿樣本中的透明質(zhì)酸 (HA) 含量而設(shè)計(jì)。
     
    1、生物學(xué)背景與研究價(jià)值:
        
    透明質(zhì)酸 (HA) 是一種廣泛分布于人體結(jié)締組織、上皮組織、神經(jīng)組織及關(guān)節(jié)滑液中的高分子糖胺聚糖,是細(xì)胞外基質(zhì)的關(guān)鍵組分。
     
    (1)生理功能:HA 具有極強(qiáng)的持水能力,能維持組織形態(tài)、潤滑關(guān)節(jié)、調(diào)節(jié)細(xì)胞遷移與增殖,并在組織修復(fù)和炎癥反應(yīng)中發(fā)揮核心作用。
    (2)臨床應(yīng)用與科研方向:
        神經(jīng)系統(tǒng)疾?。?檢測腦脊液(CSF)中的 HA 水平,輔助研究多發(fā)性硬化癥、腦損傷及神經(jīng)退行性疾病中的血腦屏障通透性與基質(zhì)重塑。
        組織再生與修復(fù): 評估 HA 在傷口愈合、皮膚年輕化及軟骨修復(fù)過程中的動態(tài)變化。
        炎癥與纖維化: 監(jiān)測血清或組織中 HA 濃度,作為肝纖維化、肺纖維化及慢性炎癥疾病的生物標(biāo)志物。
        腫瘤研究: 探究腫瘤微環(huán)境中 HA 的異常高表達(dá)對癌細(xì)胞侵襲、轉(zhuǎn)移及耐藥性的影響。
     
    2、技術(shù)參數(shù):
        檢測方法: 雙抗體夾心法 ELISA
        檢測范圍: 0.156 ng/mL - 10 ng/mL
        適用樣本: 血清、血漿、腦脊液(CSF)、組織勻漿
        適用物種: 人 (Human)

    3、應(yīng)用說明:
        本試劑盒適用于基礎(chǔ)醫(yī)學(xué)研究中 HA 代謝機(jī)制的探索、藥物干預(yù)效果的評價(jià),以及結(jié)合細(xì)胞實(shí)驗(yàn)或多組織類型樣本進(jìn)行的動態(tài)監(jiān)測。它為組織再生、炎癥調(diào)控及相關(guān)疾病機(jī)制的研究提供了高靈敏度的可靠工具。
     
    4、注意事項(xiàng):
        本品僅限科學(xué)研究使用,嚴(yán)禁用于臨床診斷。具體的實(shí)驗(yàn)操作流程、試劑配制方法及結(jié)果計(jì)算方式,請嚴(yán)格參照隨附的產(chǎn)品說明書執(zhí)行。
  • 別名:
    N/A
  • 縮寫:
    HA
  • 種屬:
    Homo sapiens (Human)
  • 反應(yīng)時(shí)間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Cell Biology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human HA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:20 Average % 87  
    Range % 80-94  
    1:40 Average % 92  
    Range % 88-99  
    1:80 Average % 97  
    Range % 91-100  
    1:160 Average % 101  
    Range % 97-105  
  • 回收率:
    The recovery of human HA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 100 95-105  
    EDTA plasma (n=4) 93 88-98  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.727 2.700 2.714 2.626  
    5 2.231 2.345 2.288 2.200  
    2.5 1.558 1.655 1.607 1.519  
    1.25 0.831 0.901 0.866 0.778  
    0.625 0.477 0.451 0.464 0.376  
    0.312 0.301 0.322 0.312 0.224  
    0.156 0.189 0.174 0.182 0.094  
    0 0.087 0.089 0.088    
  • 本試劑盒所含材料:
      • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human HA antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • Biotin-labeled HA antibody(100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
      • HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
      • HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
      • Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration.
      • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances.
      • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

    顯示更多

    收起更多

  • 本試劑盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

    顯示更多

    收起更多

  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價(jià)

平均分:
5.0分 - 2 個(gè)評價(jià)

樣品類型:細(xì)胞培養(yǎng)上清液

樣品信息:人

稀釋比:1:100

產(chǎn)品評價(jià): 我用CSB-E04805h檢測血清樣本中HA含量,試劑盒靈敏度高,數(shù)據(jù)理想。

By 張老師

樣品類型:血漿(抗凝劑) edta

樣品信息:人

稀釋比:1:100

產(chǎn)品評價(jià): 我們用CSB-E04805h檢測人冠心病患者血漿臨床樣本,檢測后的標(biāo)曲擬合結(jié)果達(dá)到0.999,復(fù)孔的CV%變異系數(shù)<10,效果較好,使用方便,為實(shí)驗(yàn)提供了有力的支持

By 類老師

相關(guān)問答

 常見問題解答
Q:

We are looking into the best method to distinguish HA manufactured by fermentation and nature human HA.

A:
Thanks for your inquiry.
CSB-E04805h Human Hyaluronic acid, HA ELISA Kit
This kit can detect nature HA in polymerized form. As for HA manufactured by fermentation, it usually exists in free form.
We think it can not be detected out by the kit.

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    HA(透明質(zhì)酸)是一種大分子多糖,廣泛存在于人體組織中。最近的研究表明,HA在細(xì)胞間通訊、細(xì)胞遷移和組織再生中發(fā)揮重要作用。HA在軟骨組織再生和修復(fù)中的應(yīng)用也受到廣泛關(guān)注。研究人員正在開發(fā)一些基于HA的藥物和材料,用于治療各種軟骨損傷和疾病,如骨關(guān)節(jié)炎、肩周炎和韌帶損傷等。


×