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  4. Human Epinephrine/Adrenaline,EPI ELISA Kit

Human Epinephrine/Adrenaline,EPI ELISA Kit

  • 中文名稱：
    人腎上腺素(EPI)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08677h
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    2 pg/mL-400 pg/mL
  • 靈敏度：
    1 pg/mL
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人腎上腺素(EPI)酶聯(lián)免疫試劑盒（CSB-E08677h）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的EPI含量。試劑盒檢測范圍為2 pg/mL-400 pg/mL，適用于神經(jīng)生物學(xué)、內(nèi)分泌學(xué)及藥理學(xué)等領(lǐng)域的基礎(chǔ)研究，例如應(yīng)激狀態(tài)下激素分泌機(jī)制探索、心血管疾病模型中的激素動態(tài)監(jiān)測，或藥物干預(yù)對腎上腺功能影響的評價(jià)等科研場景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    EPI
  • 種屬：
    Homo sapiens (Human)
  • 反應(yīng)時(shí)間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human EPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 87-98  
    1:2 Average % 95  
    Range % 90-99  
    1:4 Average % 90  
    Range % 85-96  
    1:8 Average % 90  
    Range % 84-98  
  • 回收率：
    The recovery of human EPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 87 80-94  
    EDTA plasma (n=4) 98 92-107  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    400 0.213 0.210 0.212    
    100 0.408 0.391 0.400    
    32 0.613 0.631 0.622    
    8 1.062 1.003 1.033    
    2 1.593 1.516 1.555    
    0 2.467 2.412 2.440    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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