Human Creatine Kinase MB isoenzyme,CK-MB ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (8) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人肌酸激酶同工酶MB(CK-MB)酶聯(lián)免疫試劑盒（CSB-E05140h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、尿液、組織勻漿樣本中的CK-MB含量。CK-MB即肌酸激酶同工酶，主要存在于心肌中。它是診斷急性心肌梗死等心臟疾病的重要生物標(biāo)志物。研究機(jī)制是當(dāng)心肌受損時(shí)，CK-MB會(huì)釋放進(jìn)入血液，通過(guò)檢測(cè)血中其含量變化，可輔助判斷心肌損傷程度、范圍及病情發(fā)展。試劑盒檢測(cè)范圍為0.9 ng/mL-60 ng/mL，本試劑盒適用于科研領(lǐng)域中心肌損傷相關(guān)疾病模型的機(jī)制探索，如心肌梗死、心肌炎或藥物心臟毒性評(píng)估等研究場(chǎng)景，可通過(guò)檢測(cè)不同樣本類(lèi)型（如血液樣本動(dòng)態(tài)監(jiān)測(cè)病程進(jìn)展，尿液樣本探索新型標(biāo)志物潛力，或組織勻漿分析離體心肌損傷）為心血管病理研究提供定量數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

N/A
縮寫(xiě)：

CK-MB
種屬：

Homo sapiens (Human)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-91
1:2	Average %	94
	Range %	89-100
1:4	Average %	98
	Range %	91-104
1:8	Average %	106
	Range %	102-112

回收率：

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
60	3.334	3.234	3.284	3.168
30	2.152	2.067	2.110	1.994
15	1.214	1.168	1.191	1.075
7.5	0.743	0.729	0.736	0.620
3.75	0.443	0.451	0.447	0.331
1.8	0.289	0.273	0.281	0.165
0.9	0.198	0.187	0.192	0.077
0	0.113	0.118	0.116