Human Collagen Type Ⅲ,Col Ⅲ ELISA KIT
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中文名稱:人Ⅲ型膠原(Col Ⅲ)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E04799h-IS
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:4.69 ng/mL-300 ng/mL
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靈敏度:2.345 ng/mL
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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促銷:
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其他:
產(chǎn)品詳情
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別名:Collagen Type 3,Col 3,Collagen Type III,Col III
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縮寫:Col Ⅲ
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種屬:Homo sapiens (Human)
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反應(yīng)時(shí)間:1-5h
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上樣量:50-100ul
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檢測(cè)波長:450 nm
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研究領(lǐng)域:Signal Transduction
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
98
Range %
91-103
1:2
Average %
93
Range %
88-100
1:4
Average %
104
Range %
96-110
1:8
Average %
88
Range %
81-98
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回收率:
The recovery of human Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
94
89-100
EDTA plasma (n=4)
99
93-103
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml
OD1
OD2
Average
Corrected
300
3.149
3.289
3.219
3.150
150
2.182
2.139
2.160
2.091
75
1.094
1.147
1.121
1.052
37.5
0.631
0.632
0.631
0.563
18.75
0.359
0.355
0.357
0.288
9.38
0.225
0.233
0.229
0.160
4.69
0.156
0.154
0.155
0.086
0
0.070
0.067
0.069
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數(shù)據(jù)處理:
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貨期:3-5 working days
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