Human 17-Hydroxyprogesterone,17-OHP ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人17羥孕酮(17-OHP)酶聯(lián)免疫試劑盒（CSB-E05087h）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的17-OHP含量。17-OHP即17-羥孕酮，與先天性腎上腺皮質(zhì)增生癥等疾病密切相關(guān)。其背景在于它是腎上腺皮質(zhì)激素合成過程的中間產(chǎn)物。研究機(jī)制主要聚焦于通過檢測其在血液中的水平來輔助診斷相關(guān)疾病，為臨床治療提供重要依據(jù)。試劑盒檢測范圍為0.625 ng/ml-10 ng/ml，靈敏度為0.625ng/ml。適用于內(nèi)分泌疾病機(jī)制研究、激素代謝通路探索、藥物干預(yù)效果評估等科研領(lǐng)域，特別在腎上腺相關(guān)動(dòng)物模型構(gòu)建、類固醇激素代謝組學(xué)分析及生殖內(nèi)分泌研究中可提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

17-OHP
種屬：

Homo sapiens (Human)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Biochemicals
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human 17-OHP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-103
1:2	Average %	91
	Range %	86-96
1:4	Average %	94
	Range %	89-100
1:8	Average %	89
	Range %	85-95

回收率：

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average
10	0.299	0.314	0.306
5	0.427	0.407	0.417
2.5	0.628	0.612	0.620
1.25	0.988	0.942	0.965
0.625	1.604	1.611	1.607
0	2.720	2.873	2.797