Horse Gelsolin(GSN) ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

馬凝溶膠蛋白(GSN)酶聯(lián)免疫試劑盒（CSB-EL009965HO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GSN含量。GSN 是一個靶點(diǎn)。它在細(xì)胞中具有重要作用，與細(xì)胞的運(yùn)動、形態(tài)維持等相關(guān)。研究機(jī)制方面，其可能參與調(diào)節(jié)細(xì)胞骨架的動態(tài)變化。通過對它的深入研究，有望揭示疾病發(fā)生發(fā)展機(jī)制，為開發(fā)新的治療方法和藥物提供方向。試劑盒檢測范圍為12.5 ng/mL-800 ng/mL，該產(chǎn)品可為馬屬動物細(xì)胞遷移機(jī)制、炎癥相關(guān)疾病模型或肌肉組織再生等基礎(chǔ)研究提供關(guān)鍵數(shù)據(jù)支持，適用于實(shí)驗(yàn)室條件下對生物樣本中GSN蛋白表達(dá)水平的動態(tài)監(jiān)測及比較分析，為探索其分子功能及調(diào)控機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

GSNGelsolin ELISA kit; Actin-depolymerizing factor ELISA kit; ADF ELISA kit; Brevin ELISA kit
縮寫：

GSN
Uniprot No.：

Q28372
種屬：

Equus caballus (Horse)
反應(yīng)時間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse GSN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	101
	Range %	94-105
1:2000	Average %	89
	Range %	82-94
1:4000	Average %	102
	Range %	98-106
1:8000	Average %	95
	Range %	91-99

回收率：

The recovery of horse GSN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-97
EDTA plasma (n=4)	91	87-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
基因功能參考文獻(xiàn)：
1. Ca2+ binding by domain 2 plays a critical role in the activation and stabilization of gelsolin PMID: 19666512
亞細(xì)胞定位：

Cytoplasm, cytoskeleton.
蛋白家族：

Villin/gelsolin family
數(shù)據(jù)庫鏈接：

KEGG: ecb:100034186

UniGene: Eca.2517