Hamster adiponectin, C1Q and collagen domain containing (ADIPOQ)ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

倉鼠脂聯(lián)素，含C1Q和膠原域(ADIPOQ)酶聯(lián)免疫試劑盒（CSB-EL001366HA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ADIPOQ含量。ADIPOQ是脂聯(lián)素基因。脂聯(lián)素由脂肪組織分泌，具有抗糖尿病、抗炎、抗動脈粥樣硬化等作用。研究機制上，它可與多種受體結合，激活下游信號通路，調節(jié)能量代謝、改善胰島素敏感性，在代謝性疾病等研究中受關注。試劑盒檢測范圍為0.469 ng/mL-30 ng/mL，該產品適用于科研實驗中評估倉鼠模型在代謝疾病干預、脂肪組織功能研究、運動生理學或藥物開發(fā)過程中ADIPOQ表達水平的變化，為探索脂聯(lián)素在能量代謝調控機制提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
縮寫：

ADIPOQ
Uniprot No.：

C9DFG2
種屬：

Mesocricetus auratus (Golden hamster)
反應時間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of hamster ADIPOQ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	85
	Range %	82-90
1:2000	Average %	93
	Range %	90-96
1:4000	Average %	89
	Range %	84-93
1:8000	Average %	94
	Range %	90-98

回收率：

The recovery of hamster ADIPOQ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-92
EDTA plasma (n=4)	95	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.