Goat Luteinizing Hormone (LH) ELISA KIT

產(chǎn)品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

山羊黃體生成素(LH)酶聯(lián)免疫試劑盒（CSB-E13274G）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LH含量。LH即促黃體生成素，是腺垂體分泌的促性腺激素。其在調(diào)節(jié)性腺功能方面起關(guān)鍵作用，可刺激卵巢排卵和黃體形成，促進雄激素合成及精子生成。目前對其分泌調(diào)控、與相關(guān)疾病關(guān)系等機制是研究熱點，以助解決生殖等方面問題。試劑盒檢測范圍為0.3 mLU/mL-60 mLU/mL，適用于山羊繁殖生理機制研究、卵巢功能評估、生殖內(nèi)分泌調(diào)控實驗以及繁殖障礙模型的建立等科研場景，例如通過動態(tài)監(jiān)測發(fā)情周期中LH濃度波動，探究排卵誘導(dǎo)機制或評估激素干預(yù)效果本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

LH
種屬：

Capra hircus (Goat)
反應(yīng)時間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	90-104
1:2	Average %	95
	Range %	90-103
1:4	Average %	91
	Range %	85-96
1:8	Average %	90
	Range %	88-93

回收率：

The recovery of goat LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-101
EDTA plasma (n=4)	92	87-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.