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  4. Fish Interleukin 6,IL-6 ELISA Kit

Fish Interleukin 6,IL-6 ELISA Kit

  • 中文名稱：
    魚(yú)白介素6(IL-6)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13258Fh
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    12.5 pg/mL-200 pg/mL
  • 靈敏度：
    12.5 pg/mL
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    魚(yú)白介素6(IL-6)酶聯(lián)免疫試劑盒（CSB-E13258Fh）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL-6含量。IL-6是重要的炎癥細(xì)胞因子，在免疫調(diào)節(jié)、造血等生理過(guò)程中發(fā)揮關(guān)鍵作用。異常升高時(shí)與多種疾病如自身免疫病、腫瘤等的發(fā)生發(fā)展密切相關(guān)。其研究機(jī)制主要聚焦于阻斷IL-6與其受體結(jié)合，以抑制下游信號(hào)通路從而達(dá)到治療目的。試劑盒檢測(cè)范圍為12.5 pg/mL-200 pg/mL，本試劑盒適用于水產(chǎn)養(yǎng)殖中魚(yú)類健康監(jiān)測(cè)、病原感染模型建立、免疫增強(qiáng)劑效果評(píng)估等科研場(chǎng)景，亦可用于比較醫(yī)學(xué)中脊椎動(dòng)物免疫系統(tǒng)的進(jìn)化機(jī)制探究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    il6Interleukin-6 ELISA Kit; IL-6 ELISA Kit
  • 縮寫：
    IL-6
  • 種屬：
    Fish
  • 反應(yīng)時(shí)間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 88-97  
    1:2 Average % 89  
    Range % 82-94  
    1:4 Average % 103  
    Range % 94-107  
    1:8 Average % 96  
    Range % 90-101  
  • 回收率：
    The recovery of fish IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 100 91-104  
    EDTA plasma (n=4) 91 84-98  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    200 0.128 0.133 0.131    
    100 0.269 0.258 0.264    
    50 0.449 0.431 0.440    
    25 0.851 0.842 0.847    
    12.5 1.813 1.783 1.798    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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