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Fish Cytochrome P450 1A1(CYP1A1) ELISA kit

  • 中文名稱:
    魚(yú)細(xì)胞色素P450 1A1(CYP1A1)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EL006395FI
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    75 pg/mL-1200 pg/mL
  • 靈敏度:
    75 pg/mL
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4200/¥3000
  • 促銷:
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    魚(yú)細(xì)胞色素P450 1A1(CYP1A1)酶聯(lián)免疫試劑盒(CSB-EL006395FI)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的CYP1A1含量。CYP1A1是一種主要的細(xì)胞色素P450酶,廣泛研究其激活致癌化合物的能力。它通過(guò)芳香烴受體(AhR)介導(dǎo)的轉(zhuǎn)錄激活,在代謝環(huán)境致癌物和膳食化合物中發(fā)揮重要作用。CYP1A1在肺癌等癌癥的發(fā)生發(fā)展中扮演關(guān)鍵角色,其研究機(jī)制涉及致癌物激活、基因多態(tài)性、以及與AhR等受體的相互作用。試劑盒檢測(cè)范圍為75 pg/mL-1200 pg/mL,適用于科研場(chǎng)景中評(píng)估魚(yú)類暴露于環(huán)境毒素后的生理響應(yīng)、污染物生物有效性研究以及生態(tài)毒理學(xué)模型的建立。為水生生態(tài)系統(tǒng)健康監(jiān)測(cè)、污染物代謝機(jī)制研究及水產(chǎn)養(yǎng)殖安全評(píng)估提供可靠工具本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名:
    cyp1a1 ELISA kit; cyp1aCytochrome P450 1A1 ELISA kit; EC 1.14.14.1 ELISA kit; CYPIA1 ELISA kit
  • 縮寫(xiě):
    CYP1A1
  • Uniprot No.:
  • 種屬:
    Fish
  • 反應(yīng)時(shí)間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Cancer
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of fish CYP1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 86-98  
    1:2 Average % 100  
    Range % 92-107  
    1:4 Average % 85  
    Range % 80-91  
    1:8 Average % 88  
    Range % 83-96  
  • 回收率:
    The recovery of fish CYP1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 89 88-95  
    EDTA plasma (n=4) 94 91-97  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1200 0.146 0.147 0.147    
    600 0.238 0.254 0.246    
    300 0.524 0.553 0.539    
    150 1.112 1.135 1.124    
    75 2.338 2.277 2.308    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
  • 亞細(xì)胞定位:
    Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
  • 蛋白家族:
    Cytochrome P450 family
  • 數(shù)據(jù)庫(kù)鏈接:


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