Dog Interleukin 1β (IL- 1β) ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬白介素1β(IL-1β)酶聯(lián)免疫試劑盒（CSB-E13836c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IL1B含量。IL1B，即白介素-1β，是一種強效的促炎細(xì)胞因子，主要由單核細(xì)胞、巨噬細(xì)胞和樹突狀細(xì)胞產(chǎn)生。它在炎癥和免疫反應(yīng)中起關(guān)鍵作用，通過激活I(lǐng)L-1受體和下游信號通路，調(diào)節(jié)細(xì)胞增殖、分化和凋亡。IL1B的研究對于理解炎癥性疾病的發(fā)生機(jī)制和開發(fā)治療策略具有重要意義。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，該產(chǎn)品適用于科研領(lǐng)域，如探究犬類模型中的炎癥性疾病病理過程、評估免疫調(diào)節(jié)劑或抗炎藥物的干預(yù)效果，以及動物疫苗研發(fā)中的免疫應(yīng)答研究，為獸醫(yī)學(xué)和比較醫(yī)學(xué)領(lǐng)域的實驗提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

IL1BInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
縮寫：

IL1B
Uniprot No.：

Q28292
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
反應(yīng)時間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	83-100
1:2	Average %	94
	Range %	91-99
1:4	Average %	95
	Range %	90-105
1:8	Average %	94
	Range %	86-100

回收率：

The recovery of dog IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-102
EDTA plasma (n=4)	97	90-104

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
亞細(xì)胞定位：

Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
蛋白家族：

IL-1 family
數(shù)據(jù)庫鏈接：

STRING: 9615.ENSCAFP00000010762

UniGene: Cfa.33592