Chicken Interleukin 17,IL-17 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (6) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

雞白介素17(IL-17)酶聯(lián)免疫試劑盒（CSB-E04607Ch）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL17含量。IL-17是一種由Th17細(xì)胞分泌的促炎細(xì)胞因子，參與調(diào)節(jié)免疫反應(yīng)和炎癥過(guò)程。其在銀屑病、類(lèi)風(fēng)濕關(guān)節(jié)炎等自身免疫疾病中起重要作用，同時(shí)也是腫瘤發(fā)生和進(jìn)展的關(guān)鍵因子。研究IL-17機(jī)制有助于開(kāi)發(fā)針對(duì)相關(guān)疾病的治療方法。試劑盒檢測(cè)范圍為150 pg/mL-6000 pg/mL，適用于家禽免疫學(xué)研究領(lǐng)域，如探究病原體感染后宿主免疫應(yīng)答動(dòng)態(tài)、評(píng)估疫苗免疫效果或解析炎癥性疾病模型中的細(xì)胞因子調(diào)控網(wǎng)絡(luò)，為禽類(lèi)基礎(chǔ)免疫機(jī)制研究及疾病防控提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

IL-17
Uniprot No.：

Q7T1P7
種屬：

Gallus gallus (Chicken)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	82-100
1:2	Average %	95
	Range %	90-100
1:4	Average %	96
	Range %	90-101
1:8	Average %	91
	Range %	85-98

回收率：

The recovery of chicken IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-96
EDTA plasma (n=4)	92	86-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.