Chicken Ghrelin ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (4) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

雞饑餓激素酶聯(lián)免疫試劑盒（CSB-E14230C）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的Ghrelin含量。Ghrelin是一種重要靶點(diǎn)。它是胃內(nèi)產(chǎn)生的肽，能調(diào)節(jié)食欲和能量穩(wěn)態(tài)。研究機(jī)制方面，Ghrelin與受體結(jié)合后，作用于神經(jīng)內(nèi)分泌系統(tǒng)，影響下丘腦信號(hào)通路，調(diào)節(jié)攝食、生長(zhǎng)激素釋放等，在肥胖、代謝疾病等研究中受關(guān)注。試劑盒檢測(cè)范圍為25 pg/mL-400 pg/mL，適用于家禽生理學(xué)研究、營(yíng)養(yǎng)代謝實(shí)驗(yàn)、生長(zhǎng)調(diào)控機(jī)制探索等科研領(lǐng)域，如評(píng)估飼料成分對(duì)Ghrelin分泌的影響或分析激素水平與生長(zhǎng)性能的關(guān)聯(lián)性，為禽類生物學(xué)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

Ghrelin
種屬：

Gallus gallus (Chicken)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Metabolism
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken ghrelin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-96
1:2	Average %	90
	Range %	86-95
1:4	Average %	95
	Range %	90-100
1:8	Average %	97
	Range %	92-103

回收率：

The recovery of chicken ghrelin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-98
EDTA plasma (n=4)	93	88-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.