Canine Matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬基質(zhì)金屬蛋白酶2/明膠酶A(MMP-2/Gelatinase A)酶聯(lián)免疫試劑盒（CSB-E14056c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的MMP2/Gelatinase A含量。MMP2/Gelatinase A即基質(zhì)金屬蛋白酶 2，在細(xì)胞外基質(zhì)降解和重構(gòu)中起關(guān)鍵作用。其異常表達(dá)與腫瘤生長(zhǎng)、侵襲、轉(zhuǎn)移等密切相關(guān)。研究主要圍繞其表達(dá)調(diào)控機(jī)制、與其他分子相互作用等，以探索開發(fā)針對(duì)它的治療癌癥等疾病的藥物。試劑盒檢測(cè)范圍為4.69 ng/mL-300 ng/mL，適用于犬類疾病模型研究中MMP-2動(dòng)態(tài)變化的監(jiān)測(cè)，如腫瘤侵襲轉(zhuǎn)移機(jī)制分析、傷口愈合評(píng)估或心血管疾病相關(guān)基質(zhì)重塑研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

MMP2/Gelatinase A
Uniprot No.：

F1PMK7
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cancer
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-106
1:2	Average %	85
	Range %	80-95
1:4	Average %	95
	Range %	92-100
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of canine MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-101
EDTA plasma (n=4)	92	87-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.