Canine Interferon alpha-1/2 ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬α-1/2干擾素(IFN-α1/2)酶聯(lián)免疫試劑盒（CSB-E09849c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IFN-A1/2含量。IFN-A1/2屬干擾素家族，在機體免疫防御中起關鍵作用。研究發(fā)現(xiàn)其能激活細胞內(nèi)信號通路，誘導抗病毒蛋白表達以抵御病毒感染。還參與調(diào)節(jié)免疫細胞活性和功能，在對抗腫瘤及自身免疫病中展現(xiàn)潛在價值，是研究熱點。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，適用于科研場景中犬類I型干擾素的功能研究；其動態(tài)水平變化常被用于評估病毒感染模型、疫苗免疫原性實驗或免疫調(diào)節(jié)相關機制研究；細胞培養(yǎng)上清液可分析體外刺激后免疫細胞分泌情況，組織勻漿則適用于疾病模型中局部干擾素水平的定量分析；為動物免疫學、病毒學及炎癥機制研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Interferon alpha-1/2 ELISA Kit; IFN-alpha-1/2 ELISA Kit
縮寫：

IFN-α1/2
Uniprot No.：

P81255
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
反應時間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine IFN-α1/2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	86-99
1:2	Average %	100
	Range %	94-109
1:4	Average %	92
	Range %	89-96
1:8	Average %	87
	Range %	83-96

回收率：

The recovery of canine IFN-α1/2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-96
EDTA plasma (n=4)	94	88-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
亞細胞定位：

Secreted.
蛋白家族：

Alpha/beta interferon family
數(shù)據(jù)庫鏈接：

KEGG: cfa:100685213

UniGene: Cfa.4550