Bovine secretory immunoglobulin A,sIgA ELISA Kit

產(chǎn)品詳情

說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

牛分泌型免疫球蛋白A(sIgA)酶聯(lián)免疫試劑盒（CSB-E11192b）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)唾液樣本中的sIgA含量。sIgA即分泌型免疫球蛋白 A，是黏膜免疫系統(tǒng)的關(guān)鍵效應(yīng)分子，在抵御病原體入侵方面發(fā)揮重要作用。其能阻止病原體黏附于上皮細(xì)胞，中和毒素。研究機(jī)制著重于其產(chǎn)生調(diào)控、與微生物相互作用及在疾病發(fā)生發(fā)展中的變化。試劑盒檢測(cè)范圍為2.4 μg/mL-150 μg/mL，適用于科研領(lǐng)域中對(duì)牛黏膜免疫功能的評(píng)估，如病原體感染機(jī)制研究、疫苗免疫效果監(jiān)測(cè)、飼料添加劑對(duì)黏膜免疫的影響分析等場(chǎng)景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
縮寫：

sIgA
種屬：

Bos taurus (Bovine)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	92
	Range %	85-101
1:40	Average %	95
	Range %	90-99
1:80	Average %	93
	Range %	85-97
1:160	Average %	97
	Range %	92-110

回收率：

The recovery of bovine sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	94-100
EDTA plasma (n=4)	94	87-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.