Bovine Thrombin-antithrombin III Complex, TAT-III ELISA Kit

說明書下載

產(chǎn)品詳情

產(chǎn)品描述：

牛凝血酶抗凝血酶Ⅲ復(fù)合物(TAT-III)酶聯(lián)免疫試劑盒（CSB-E14133B）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的TAT-III含量。試劑盒檢測(cè)范圍為1.56 pg/mL-100 pg/mL，本試劑盒可應(yīng)用于心血管疾病動(dòng)物模型研究、凝血相關(guān)藥物開發(fā)中的藥效評(píng)價(jià)，或探索凝血 - 抗凝系統(tǒng)動(dòng)態(tài)平衡的分子調(diào)控機(jī)制，為科研領(lǐng)域提供靈敏、特異的檢測(cè)方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

TAT-III
種屬：

Bos taurus (Bovine)
反應(yīng)時(shí)間：

1-5h
上樣量：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine TAT-III in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	92
	Range %	88-96
1:40	Average %	85
	Range %	80-90
1:80	Average %	104
	Range %	100-110
1:160	Average %	97
	Range %	92-101

回收率：

The recovery of bovine TAT-III spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	89	85-93

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.