Bovine Interleukin 2,IL-2 ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

別名：

IL2 ELISA kit; IL-2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
縮寫：

IL2
Uniprot No.：

P05016
種屬：

Bos taurus (Bovine)
反應(yīng)時間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-97
1:2	Average %	99
	Range %	92-106
1:4	Average %	94
	Range %	89-99
1:8	Average %	101
	Range %	97-105

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
10	3.011	2.934	2.972	2.849
5	1.914	1.967	1.940	1.817
2.5	1.050	1.028	1.039	0.916
1.25	0.615	0.609	0.612	0.489
0.625	0.288	0.301	0.295	0.171
0.312	0.181	0.191	0.186	0.063
0.156	0.146	0.154	0.150	0.027
0	0.121	0.125	0.123

功能：

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
基因功能參考文獻：
1. Pretreatment of neonatal PBMC with IL-1beta, TNF-alpha or IFN-gamma promotes mitogenic response to ConA through up-regulating the production of IL-2 and the expression of the mature IL-2 receptor. PMID: 12906107
亞細胞定位：

Secreted.
蛋白家族：

IL-2 family
數(shù)據(jù)庫鏈接：

KEGG: bta:280822

STRING: 9913.ENSBTAP00000027819

UniGene: Bt.341