Bovine Beta-Hydroxybutyric Acid,β-OHB ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

牛β羥丁酸(βOHB)酶聯(lián)免疫試劑盒（CSB-E10056b）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的β-OHB含量。β-OHB即β-羥基丁酸，是酮體的主要成分之一。它在人體代謝中發(fā)揮著重要作用，可作為能量底物供能。研究發(fā)現(xiàn)其能通過調(diào)節(jié)多種信號通路，參與細(xì)胞代謝、炎癥反應(yīng)、氧化應(yīng)激等過程，在神經(jīng)保護(hù)、代謝性疾病等方面研究較多。試劑盒檢測范圍為50 nmol/mL-800 nmol/mL，適用于畜牧科研領(lǐng)域，例如探究牛群能量代謝特征、營養(yǎng)調(diào)控對酮體生成的影響，或作為代謝綜合征模型的生物標(biāo)志物檢測工具。實驗操作兼容常規(guī)實驗室設(shè)備，為動物營養(yǎng)學(xué)、獸醫(yī)學(xué)研究提供高效、穩(wěn)定的檢測方案，數(shù)據(jù)結(jié)果僅用于科學(xué)研究，不涉及其他用途。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

beta-Hydroxybutyric acid, beta-OHB
縮寫：

β-OHB
種屬：

Bos taurus (Bovine)
反應(yīng)時間：

1-5h
上樣量：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine β-OHB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	88-95
1:2	Average %	96
	Range %	91-103
1:4	Average %	98
	Range %	92-104
1:8	Average %	95
	Range %	89-100

回收率：

The recovery of bovine β-OHB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	90-103
EDTA plasma (n=4)	95	90-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.